• JIM THOMAS
• SURESHKUMAR M
• VASTHI KENNEDY EVANJELENE

Natural products from medicinal plants, either as pure compounds or as standardized extracts, provide unlimited opportunities for new drug leads because of the unmatched availability of chemical diversity. Due to an increasing demand for chemical diversity in screening programs, seeking therapeutic drugs from natural products, interest particularly in edible, has grown throughout the world. Botanicals and herbal preparations for medicinal usage contain various types of bioactive compounds. Fractional analysis of these bioactive compounds have yielded several innovative compounds of pharmaceutical and biomedical importance. This study is focused on step-by-step visual demonstration of fractionation and isolation of biologically active plant secondary metabolites using column-chromatographic techniques. Isolation of bioactive compounds using column-chromatography involves: a) Preparation of sample; b) Packing of column; c) Pouring of sample into the column; d) Elution of fractions; and e) Analysis of each fractions using thin layer chromatography. The analysis of bioactive compounds present in the plant extracts involving the applications of common phytochemical screening assays, chromatographic techniques such as TLC as well as non-chromatographic techniques such as Immunoassay, Nuclear Magnetic Resonance (NMR) spectral analyses and Fourier Transform Infra Red (FTIR) analyses are discussed.

Bioactive compound, Plant Extraction, Isolation, Calotropis gigantea Stem Methanolic Extract (CGSME).

"PHYTOCHEMICALS: EXTRACTION, ISOLATION AND IDENTIFICATION OF BIOACTIVE COMPOUNDS FROM CALOTROPIS GIGANTEA R.BR. (LINN) –ERUKKU", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 1, page no.659-668, January-2019, Available :http://www.jetir.org/papers/JETIR1901992.pdf

"PHYTOCHEMICALS: EXTRACTION, ISOLATION AND IDENTIFICATION OF BIOACTIVE COMPOUNDS FROM CALOTROPIS GIGANTEA R.BR. (LINN) –ERUKKU", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 1, page no. pp659-668, January-2019, Available at : http://www.jetir.org/papers/JETIR1901992.pdf