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Published in:

Volume 8 Issue 3
March-2019
eISSN: 2349-5162

Unique Identifier

JETIR1903L34

Page Number

241-248

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Title

'Production and Partial Purification of Arginine Deiminase Isolated from Bacteria'

ISSN

2349-5162

Cite This Article

"'Production and Partial Purification of Arginine Deiminase Isolated from Bacteria'", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 3, page no.241-248, March-2019, Available :http://www.jetir.org/papers/JETIR1903L34.pdf

Abstract

An arginine deiminase (EC 3.5.3.6)is an hydrolytic enzyme that catalyzes the arginine and produce citruline as a product. Hence it has been studied as a potential anti-tumor drug for the treatment of arginine-auxotrophic tumors, in the treatment of tumors susceptible to arginine deprivation, such as malignant melanoma and hepatocellular carcinoma, arginine deiminase (AD), an arginine-degrading enzyme, has been used. In addition, tests of human lymphatic leukemia cell lines show that ADI is a potential anti-angiogenic agent and is effective in leukemia therapy. In a broad range of microorganisms, including bacteria, actinomycetes, yeast and fungi, ADI is present throughout nature. Soil samples for the isolation of bacteria have been used in recent research work. . 11 bacteria isolates were collected and tested for ADI, activity was only seen in 2 isolates labeled ADI 9 and ADI 11. In order to verify the efficiency of ADI production, these 2 isolates were further screened. ADI 9 provided the highest ADI enzyme (83 IU/ml). Via ammonium sulpate precipitation and dialysis, partial enzyme purification was performed. It was found that the optimum pH was 7, the optimum temperature was 37oC and the maximum enzyme production of glucose as a carbon source was provided by the effect of pH, temperature and carbon sources on the enzyme. ADI 9 isolate was identified with the help of Gram character, morphological character and biochemical test. The ADI 9 bacteria have been shown to belong to Bacillus sps.

Key Words

Arginine deiminase (ADI), Anticancer, dialysis, Bacillus sps.

Cite This Article

"'Production and Partial Purification of Arginine Deiminase Isolated from Bacteria'", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 3, page no. pp241-248, March-2019, Available at : http://www.jetir.org/papers/JETIR1903L34.pdf

Publication Details

Published Paper ID: JETIR1903L34
Registration ID: 306462
Published In: Volume 8 | Issue 3 | Year March-2019
DOI (Digital Object Identifier):
Page No: 241-248
ISSN Number: 2349-5162

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Cite This Article

"'Production and Partial Purification of Arginine Deiminase Isolated from Bacteria'", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 3, page no. pp241-248, March-2019, Available at : http://www.jetir.org/papers/JETIR1903L34.pdf




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