• Sumeet Singh
• Soha Patil
• Siddhi Sitap
• Aaradhika Menon
• Dr. Shruti Singh

The methanol extracts of Ocimum tenuiflorum , Azadirachta indica ,Mangifera indica ,Allium sativum, were screened for their antimicrobial activity. The test organisms included bacteria (S.aureus), and fungi (C. albicans). A simple bioautographic procedure, involving spraying suspensions of the bacteria or fungi on thin layer chromatography (TLC) plates developed in solvents of varying polarities was used to detect the number of antibacterial and antifungal compounds present in the extracts. All the extracts had antimicrobial activity against at least one of the test microorganisms. This activity was denoted by white spots against a red-purple background on the TLC plates after spraying with tetrazolium salt. The bacteria tested, S. aureus was inhibited by the most compounds separated on the TLC plates from all the tested plants. Similarly, growth of the fungus C. albicans was also inhibited by many compounds present in the extracts. Ocimum tenuiflorum , Azadirachta indica appeared to be the plant extract with the highest number of inhibition area when compared with other plants Qualitative phytochemical analysis demonstrated the presence of flavonoids and alkaloids in all plants tested. Thin layer chromatography bioautography assay of methanolic extracts of plants indicated flavonoids, alkaloids and tannins as major active compounds against Staphylococcus aureus and Candida albicans. Antimicrobial agar well assay revealed that all plants extract contain potent antimicrobial property against both the tested organisms with more zone of inhibition for C.albicans than S.aureus.

Key words: Bioautography, Medicinal Plants, Phytochemical Analysis, Agar Well Diffusion.

" STUDIES ON SCREENING OF ANTIMICROBIALS OF SOME MEDICINAL PLANTS BY BIOAUTOGRAPHY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.303-307, May-2019, Available :http://www.jetir.org/papers/JETIR1905944.pdf

" STUDIES ON SCREENING OF ANTIMICROBIALS OF SOME MEDICINAL PLANTS BY BIOAUTOGRAPHY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp303-307, May-2019, Available at : http://www.jetir.org/papers/JETIR1905944.pdf