• I.Shivani
• K.Gupta
• M.Arya
• S.Rai
• R.Rane

Antibiotic is one of the most important commercially exploited secondary metabolites produced by bacteria and fungi and employed in a wide range. With the increase in antibiotic resistance in microbial population the need for new antibiotics is increasing day by day. Extreme habitats are increasingly being recognized as sources of secondary metabolites which provide an encouraging source for development of novel natural pharmaceuticals. The present study is focused on screening of samples collected from under-explored ecological niches like marine environments &rhizosphere of plants. Screening of bacteria with potential antibiotic activity was carried out using Wilkins overlay technique. The isolates were identified based on their morphology & further confirmed through biochemical tests. Antibiotic producing ability was confirmed by Antimicrobial Susceptibility Testing (AST). A total of 11 isolates belonging to different genera were found. The production and extraction of antibiotics were done using synthetic fermentation broth method and methanol extraction method. The Minimal Inhibition Concentration (MIC) was determined by agar well diffusion method. UV spectrophotometric analysis was carried out to determine the class of antibiotics. Present research suggests that soil & hot water spring isolates having antibiotic producing capability can be further employed for industrial production of antibiotics after proper standardization.

Screening, Biochemical tests, Antimicrobial Susceptibility Testing (AST), Minimal Inhibition Concentration (MIC), UV spectrophotometry.

"SCREENING AND CHARACTERIZATION OF ANTIBIOTIC PRODUCERS FROM SOIL AND HOT WATER SPRINGS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.64-69, May-2019, Available :http://www.jetir.org/papers/JETIR1905I09.pdf

"SCREENING AND CHARACTERIZATION OF ANTIBIOTIC PRODUCERS FROM SOIL AND HOT WATER SPRINGS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp64-69, May-2019, Available at : http://www.jetir.org/papers/JETIR1905I09.pdf