• Aneesha A. Tankariwala
• Dr. Arti A Raval

Abstract The environmental impacts caused by plastics wastes have attracted worldwide concern. Bacteria can synthesize a wide range of bio-polymers that serve diverse biological function and have material properties suitable for numerous industrial and medical applications. Polyhydroxyalkanotes are polymers produced by bacteria, among which polyhydroxybutyrates are one major group. Since the production of bioplastics is expensive so many techniques have been adopted for its large scale production. The following study was undertaken to screen and isolate bacteria producing such compounds. Different soil and marine water samples were collected and streaked onto various agar media for isolation. Colonies showing different morphologies were selected for further studies. Their morphological biochemical characteristics were studied. The bacteria were then analyzed for the PHB granules by Carbol fuchsin and Sudan black staining. And for conformation bacteria were also cultivated in presence of Nile blue staining. The colonies that showed fluorescence were selected and were inoculated in production media containing organic wastes for the production of bio-plastics.

1. Biopolymers 2. Polyhydroxyalkanotes3. Bioplastics 4. flourescence

"Screening, Isolation, and Characterization of PHB producing bacteria and Biosynthesis of PHB using different wastes.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.664-675, May-2019, Available :http://www.jetir.org/papers/JETIR1905R98.pdf

"Screening, Isolation, and Characterization of PHB producing bacteria and Biosynthesis of PHB using different wastes.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp664-675, May-2019, Available at : http://www.jetir.org/papers/JETIR1905R98.pdf