• MUKESH KUMAR
• RAAGEEVA BIMAL

The innovative research was undertaken to study the in vitro responses of different clones of Dalbergia sissoo. For this morphogenetic studies, different clones from different shisham plants found in L. S. College, Muzaffarpur campus & University campus, Muzaffarpur(Bihar) were investigated. In this study, a standard procedure was developed for rapid multiplication of Dalbergia sissoo through stem (node & internode) proliferation. The nodal & internodal explants were collected from different clones and were sterilized with 0.1% mercuric chloride for 10 minutes. Explants were collected throughout the years and the effect of season was observed. In all the different clones, explants collected from November to February respond at higher frequency and induced more shoots at optimum requirement of each clone. In few clones, maximum response of 85% was recorded, while in few clones, it was 82%. The nodal (uni and bi) part of stem gave mass proliferation of callus with 2,4-D(1.1mg/l) and BAP (1.1 mg/l) in 12-18 days. The internodal (soft & green) parts of stem gave more mass proliferation of callus with 2,4-D (1.1 mg/l) and BAP (5.5 mg/l) in 12-25 days. With the combination of 2,4-D (1.1mg/l) and BAP (5.5mg/l) the shoot apices swell up and became fleshy. The mass callus formation was also observed. The multiple callus formation from the node and internode culture, & further their regeneration into plantlets proved that for the micropropagation the soft stem could be a suitable explant. Regenerated plants were hardened and acclimatized before field transfer.

Morphogenetic studies, Tissue culture, micropropagation, callus, clones, Dalbergia sissoo.

"Morphogenetic studies of Dalbergia sissoo: An innovative research for mass production", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.2130-2136, May-2019, Available :http://www.jetir.org/papers/JETIR1905U02.pdf

"Morphogenetic studies of Dalbergia sissoo: An innovative research for mass production", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp2130-2136, May-2019, Available at : http://www.jetir.org/papers/JETIR1905U02.pdf