• Pratima Savta Phulsagar
• Jeetendra kulkarni
• Lakshmi Velide

Spinacia Oleracea is green leafy vegetable belonging to family Amaranthaceae, subfamily Chenopodioideae. It is commonly known as Spinach or Palak. It is often used as food. It can be used as therapeutic and curative medicine against several diseases because of presence of important active phytochemicals and its antibacterial activity. The different types of leaf extracts of S. Oleracea were prepared (quath, aqueous, ethanolic extract). Antibacterial activity through agar disc diffusion was carried out. Phytochemical analysis was done for different extracts. The present study investigated the antibacterial activity of aqueous, ethanol extract at different concentrations (25%,50%,100%) along with pure extract(extract without solvent).The spinach leaf pure extract without any solvent inhibited the growth of selected bacteria Staphylococcus aureus and Klebsiella pneumoniae. The aqueous and ethanolic extract had no effect on the growth of the tested bacteria. A Phytochemical analysis of different plant extract (quath, aqueous,ethanol extracts) shows the presence of carbohydrates, protein, alkaloids, flavonoid, glycosides, cardiac glycosides, phenolic compounds, tannin, saponin, terpenoids, steroids. This study revealed that Spinach shows presence of antibacterial agents. Spinach consist of important Phytochemical constituents like alkaloids, flavonoid, phenolic compounds, tannin, saponin, cardiac glycosides etc. which suggests that Spinach is a good nutrient rich vegetable with great medicinal value.

Antibacterial Activity, Spinacia Oleracea, Phytochemical Analysis

"SCREENING OF ANTIBACTERIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF SPINACIA OLERACEA", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.367-375, May 2019, Available :http://www.jetir.org/papers/JETIR1907U78.pdf

"SCREENING OF ANTIBACTERIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF SPINACIA OLERACEA", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp367-375, May 2019, Available at : http://www.jetir.org/papers/JETIR1907U78.pdf