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"Isolation of Endophytic fungus from Catharanthus roseus (L.) G. Don. and their antibacterial activity", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 2, page no.1321-1329, February-2021, Available :http://www.jetir.org/papers/JETIR2102157.pdf

• H.JAYASUDHA
• S. FATHUNNISHA
• DR.V.HEMAMALINI
• DR. M.MENAGA
• P. SRIDHAR

Abstract Medicinal plants play an important role for health care. Medicinal have ability to cure both infectious and non-infectious diseases. . The capability of medicinal plant endophytic fungi for the production of bioactive secondary metabolites was evaluated under in vitro conditions. In the present investigation the diversity of endophytic fungi was studied associated with medicinally important plant Catharanthus roseus. A total of 14 fungal species from Catharanthus roseus were isolated and identified based on the morphology of the fungal culture and characteristics of the spores. Secondary metabolites were produce from isolated endophytic fungi to investigate their antibacterial activity. The fungal culture was extracted with ethyl acetate and crude extract was used to test against clinical pathogenic organisms. The bacterial strain used in this research work were Staphylococcus aureus (MTCCB 737), Escherichia coli (MTCCB 82), Bacillus (MTCCB 1272), Pseudomonas aeruginosa (MTCCB 741) were obtained from Microbial Type culture collection, Pune, India. Then by using agar diffusion assay antibacterial activity was estimated. The zone of inhibition were measured by scaling and represented by tables and graphs.

Keywords: Endophytic fungus, Catharanthus roseus, Disc Diffusion method, Antibacterial Activity.

"Isolation of Endophytic fungus from Catharanthus roseus (L.) G. Don. and their antibacterial activity", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 2, page no. pp1321-1329, February-2021, Available at : http://www.jetir.org/papers/JETIR2102157.pdf