• Dr. Archana Kumbar
• Dr. Ashok Kumar B N
• Dr. Radhika Ranjan Geethesh P
• Dr. Ravindra Angadi
• Dr. Sushmitha V S

ABSTRACT Introduction: Standardization is the process of evaluating quality & purity of drugs by means of various parameters. HPTLC is on among them which works on the principle of separation is adsorption. It is enhanced form of TLC. Haridradi Lepa is explained by our acharyas under granthi/ arbuda chikitsa. Aims & Objectives: To standardize and evaluate the different constituents present in Haridradi Lepa. Methodology: 3µl, 6µl, 9µl of each of the Haridradi Lepa extract was applied on a pre-coated silica gel plates to a band width of 7 mm using Linomat 5 TLC applicator. The plate was developed in Toluene: Ethyl Acetate: Formic acid (5:1.5:0.5). The developed plates were visualized in short UV, long UV and then derivatized with vanillin sulphuric acid and scanned under UV 254, 366 nm and 620nm. Rf, color of the spots and densitometric scan were recorded. Results: Under short UV it showed 1 band, under long UV 9 bands were observed. Following derivatisation vanillin sulphuric acid (VSA) showed 3 bands. When the plates were densitometrically scanned at 254nm, it showed 6 bands, at 366nm, there were 5 bands observed, at 620nm 6 peaks were observed. Conclusion: The peaks observed in HPTLC can be considered for fingerprint profile analysis.

Keywords: Haridradi Lepa, arbuda, granthi, HPTLC

"Standardisation of Haridradi Lepa using High Performance Thin Layer Chromatography ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 9, page no.c276-c280, September-2021, Available :http://www.jetir.org/papers/JETIR2109235.pdf

"Standardisation of Haridradi Lepa using High Performance Thin Layer Chromatography ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 9, page no. ppc276-c280, September-2021, Available at : http://www.jetir.org/papers/JETIR2109235.pdf