• Meghana Aradhya
• M S Siddalinga Swamy
• Anil Kumar S Manchi

A simple, accurate and precise Ratio spectra derivative approach was devised and validated to simultaneously assess Itraconazole (ITZ) and Secnidazole (SEC). The ratio derivative spectroscopic approach that has been devised comprises measuring the amplitude of the ratio spectra at 262 nm for ITZ and 311 nm for SEC. The developed method used methanol as a solvent. The Beer's law complied in the concentration range of 3–15 μg/ml and 4–20 μg/ml for ITZ and SEC respectively. The proposed approach revealed that the percentage assay fell between 99.33 and 101.66 percent for ITZ and 99.5 and 101.25 percent for SEC. The results of analysis had been statistically evaluated in compliance with ICH recommendations. Recovery studies have verified the developed procedure's accuracy as well as reproducibility. ITZ and SEC were discovered to have LOD numbers of 0.01672 μg/ml and 0.01303 g/ml. ITZ and SEC were found to have LOQ numbers of 0.05067 μg/ml and 0.03949 μg/ml respectively.

Itraconazole, Secnidazole, Ratio spectra derivative, Validation, UV spectrophotometry.

"Development and Validation of UV Spectrophotometric Method for the Simultaneous Estimation of Itraconazole and Secnidazole in Bulk and Pharmaceutical Formulations", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 7, page no.g270-g275, July-2023, Available :http://www.jetir.org/papers/JETIR2307642.pdf

"Development and Validation of UV Spectrophotometric Method for the Simultaneous Estimation of Itraconazole and Secnidazole in Bulk and Pharmaceutical Formulations", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 7, page no. ppg270-g275, July-2023, Available at : http://www.jetir.org/papers/JETIR2307642.pdf