• Prashant Kashinath Wankhede

Quercetin is flavonoid which are naturally occurring in the human diet. In plant physiology, flavonoids have a long evolutionary history. They are known to control antifungal and bactericidal functions are two further biological functions that plants have exercise Ferulic acid (FA) is a phenolic acid that is found in abundance in fruits and vegetables. Several studies have revealed that FA works as an antioxidant by scavenging free radicals and boosting the cell stress response by up-regulating cytoprotective mechanisms in recent years. Various organic solvents were tried in varied proportions then four were selected for mobile phase. The chromatographic condition suggested by the software were mobile phase of Toluene:Ethylacetate:formicacid:methanol in ratio of (7:2:0.9:0.3) and saturation time 15 min. Separation was achieved using 10.0 ×10.0 HPTLC plates silica gel 60 F254 GLP Manufacturer LOBA CHEMIE . CAMAG winCats software was used and densitometric scanning was accomplished at 366nm

Quercetin,Ferulic acid, HPTLC,Validation, mobile phase, ICH

"Analytical Method Development and Validation of High Performance Thin Layer Chromatographic Method for Simultaneous Estimation of Quercetin and Ferulic acid in Marketed Herbal Tablet Formulation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 7, page no.i409-i421, July-2023, Available :http://www.jetir.org/papers/JETIR2307857.pdf

"Analytical Method Development and Validation of High Performance Thin Layer Chromatographic Method for Simultaneous Estimation of Quercetin and Ferulic acid in Marketed Herbal Tablet Formulation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 7, page no. ppi409-i421, July-2023, Available at : http://www.jetir.org/papers/JETIR2307857.pdf