• Pooja Gopalsing Bedwal

The orange peel is used as essential oil which has a variety of uses ranging from food flavouring to cosmetics. Orange oil is extracted using a variety of processes including simple distillation and solvent extraction and also Novel techniques such as supercritical CO2 extraction and turbo distillation have also been used. Where focuses on simple distillation, which involves preheating the orange peels before distillation. The preheating of the orange peels improves the oil output. This method of extraction can also be used to extract aromatic oils. HPTLC is a versatile technique that is known for its precision and accuracy of results as well as its uniformity, purity profile, and assay values. It can handle a large number of samples of various types and compositions. HPTLC is an advanced analytical separation technology with numerous applications and saves time and money. The goal of this work is to develop a new, accurate, robust, and validated HPTLC method for limonene. The analysis was performed on a silica gel 60F254 plate with mobile phase Chloroform: Methanol (8:2 v/v ). The detection wavelength is 254 nm. The Rf value for limonene is 0.24 ± 0.05. the goal of this study was to develop such a method and validation in accordance with International Council of Harmonisation (ICH) guidelines.

"“Extraction Of Limonene From Orange Peel; Analytical Method Development And Validation By HPTLC Method”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 7, page no.j455-j470, July-2023, Available :http://www.jetir.org/papers/JETIR2307959.pdf

"“Extraction Of Limonene From Orange Peel; Analytical Method Development And Validation By HPTLC Method”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 7, page no. ppj455-j470, July-2023, Available at : http://www.jetir.org/papers/JETIR2307959.pdf