• Abhishek Deepak Dhumal
• Dr Gayatri Barabde

: A simple, specific, linear, precise and accurate reverse phase liquid chromatographic method was developed to minimize run time and retention time of Telmisartan and Hydrochlorothiazide in combination and single dosage forms. The chromatographic separation was performed using Prudent C 8 Column (60 x 3 mm, 5 μm particle size). Mobile phase used was Gradient composed of buffer and Solution A was selected and a flow rate of 1.3 ml/minute was monitored with injection volume of 5 μl. Detection was carried out at 270 nm for Hydrochlorothiazide (HCTZ) and 298 nm for Telmisartan (TMS). The method was validated as per ICH guidelines. The retention time for TMS was observed as 3.6min and for HCTZ was observed as 1.2 min. Linearity range was observed in concentration of 50 - 150 μg/ml. The percentage recovery of TMS was 99% and for HCTZ was 102.6%. The proposed method was validated and successfully applied to the estimation of Telmisartan and Hydrochlorothiazide in tablet dosage forms.

Telmisartan, Hydrochlorothiazide, method development, Validation, Force Degradation

"Stability Indicating RP-HPLC Method Development and Validation: Strategy to Minimize Run Time and Retention Time Using Telmisartan in Combination and Single Dosage form and Determine its Stability", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 8, page no.d764-d777, August-2023, Available :http://www.jetir.org/papers/JETIR2308395.pdf

"Stability Indicating RP-HPLC Method Development and Validation: Strategy to Minimize Run Time and Retention Time Using Telmisartan in Combination and Single Dosage form and Determine its Stability", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 8, page no. ppd764-d777, August-2023, Available at : http://www.jetir.org/papers/JETIR2308395.pdf