• N.KARPAGAM
• M.SATHYANATHAN
• M.ELAMVALUTHI
• J.KAVINA
• M.GOPALAKRISHNAN

The present investigation entitled “In vitro Propagation of Angeloina angutifolia Benth belongs to the family Plantanginaceae. The present work was aimed towards the development of a protocol for the large scale multiplication of this important medicinal plant. Murashige and Skoog (1962) medium, was used as a basal medium with different concentrations and combinations of growth regulators. The explants, axillary buds and shoot tips were excised from the healthy mother plant selected for initiation of shoots. Fifteen different combinations were used for the induction and multiplication of shoots; among these MS-7 fortified with 2mg/L of BAP and 0.5mg/L of IAA was found to be the most suitable one for the initiation of shoots. Highest mean number of shoots (7.10±0.50) was observed on MS-7 fortified with BAP (2mg/L) + IAA (0.5mg/L) Healthy multiple shoots were produced on multiplication phase and these shoots can be taken over for the next stage of rooting.Twelve different combinations were used for the rooting of in vitro raised shoots; among these MS-17 fortified with 1mg/L of IAA was found to be the most suitable one for the initiation of shoots. In conclusion, we improved in vitro regeneration efficiency from axillary bud and shoot tip explants of A.angustifolia on MS medium supplemented with BAP alone and in combination with (IAA and NAA). Axillary bud explants protocol to be a better one for the adventitious shoot induction and produced more number of shoots when compared to shoot tip explants. Optimal concentration of BAP (2 mg/l) along with IAA (0.5 mg/l) increases the multiple shoot induction. This standardized protocol may be helpful for large scale propagation and also for the conservation of this important medicinal, ornamental species.

Angeloina angutifolia , 2-iso Pentenyl adenine, In vitro studied, Benzyl Amino Purine, EDTA

"IN VITRO PROPAGATION OF Angelonia angustifolia BENTH .", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 8, page no.e93-e125, August-2023, Available :http://www.jetir.org/papers/JETIR2308412.pdf

"IN VITRO PROPAGATION OF Angelonia angustifolia BENTH .", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 8, page no. ppe93-e125, August-2023, Available at : http://www.jetir.org/papers/JETIR2308412.pdf