• Babita B. Nandi
• Dr.Rinkal Patel

Nanotechnology is a growing field of study in which we work with materials at nanodimensions. Metal nanoparticles are traditionally synthesised using complex and expensive tools or expensive ingredients. Furthermore, the approaches may be harmful to the environment. As a result, "green" technologies for nanoparticle creation are always favoured. This dissertation describes a "green" approach for producing silver nanoparticles. The process is easy, convenient, and environmentally beneficial. Scholars have showed a strong interest in nanotechnology in recent years. This research focuses on nanoparticles, which typically have dimensions of 1-100 nm in at least one dimension. Because of their small size and unique physical features, nanoparticles are frequently used in a variety of sectors, including medical chemistry and atomic physics. They are thought to impact the behaviour of other substances with which they come into contact. Different chemical, physical, and biological methods can be used to conveniently create these particles. The antibacterial activity of the synthesised silver nanoparticles was tested against Bacillus subtilis, Escherichia coli, and Klebisella pneumonia bacteria. The nanoparticles were antimicrobial. The effective inhibitory zone is substantially larger in E. coli and K. pnuemonia than in B. subtilis. It can be determined that nanoparticles are more effective against tested bacteria. The DPPH technique was used to investigate the anti-oxidant activity of the synthesised silver nanoparticles. Silver nanoparticles had a higher percentage of anti-oxidants than extract. Two methods were employed to evaluate the anti-inflammatory activity of the synthesised silver nanoparticles. 1. Denaturation of proteins 2. Hemolysis caused by heat. The powerful percentange was demonstrated by both ways. Silver nanoparticles have a higher percentage value than extract.Alpha amylase was used to investigate the anti-diabetic activity of the synthesised silver nanoparticles. It demonstrates the greater impact of silver nanoparticles than the extract. The presence of melanin was determined by various test that has been extracted by neem leaves. It was investigated and confirmed the existence of melanin in Azadirachta indica leaves. To determine which phytochemicals are present in neem leaves, a phytochemicals test was performed using several reagents, and it was discovered that nearly all types of phytochemicals are found in neem leaves. To determine the greatest SPR peak, UV-VIS spectroscopy of silver nanoparticles was performed within 24 hours of their synthesis.

Green leaf, Nanoparticles, Melanin, silver nanoparticles, bacteria, spectroscopy, phytochemicals, anti- oxidant, anti- inflammatory, anti- diabetic

"EXTRACTION OF MELANIN AND SYNTHESIS OF SILVER NANOPARTICLES FROM AZADIRACHTA INDICA, AS WELL AS ITS PHYTOCHEMICAL AND VARIOUS ACTIVITIES.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 8, page no.g730-g738, August-2023, Available :http://www.jetir.org/papers/JETIR2308684.pdf

"EXTRACTION OF MELANIN AND SYNTHESIS OF SILVER NANOPARTICLES FROM AZADIRACHTA INDICA, AS WELL AS ITS PHYTOCHEMICAL AND VARIOUS ACTIVITIES.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 8, page no. ppg730-g738, August-2023, Available at : http://www.jetir.org/papers/JETIR2308684.pdf