• Mrunmayee Bhatawadekar
• Shraddha Gaikar
• Prachi Hadkar
• Raunaq Hashmi
• Fatima Khan,Ruchita Rane

Single cell proteins are dried cells of microorganisms used as protein source in human and animal feed. Microorganisms such as algae, bacteria, fungi utilize cheap feedstock/waste for growth and develop biomass and protein concentrates intra cellularly. Alarmingly increasing rates of population and increased pressure on agriculture base food production urges us to search for alternative protein sources. Bacteria such as Lactobacillus species and Bacillus clausii are edible, safe and probiotic bacteria in which SCP production can be ventured. In this project Sugarcane bagasse and Fish waste were employed as carbohydrate and nitrogen source respectively. Sugarcane bagasse is a cheap and abundant lignocellulosic agricultural waste. It mainly contains cellulose (polysaccharide) which can be converted into monosaccharides like glucose through chemical treatment to be utilised as fermentable carbohydrate source. Fish waste includes skin, head, viscera, trimmings, liver, bones etc. These by products contain a good amount of protein rich material which can be converted to fish protein hydrolysates (FPH). This hydrolysate acts as great nitrogen source A submerged fermentation of these bacterial species at room temperature with aeration gave sufficient product over the span of 7 days. Both the bacteria produced higher amounts of intracellular protein when grown on media formulated with sugarcane bagasse extract and fish waste hydrolysate compared to the synthetic growth media. Hence agricultural and fish industry waste can be utilised into value added product like SCP.

Single cell protein, sugarcane bagasse, fish viscera, fermentation, Bacillus clausii, Lactobacillus.

"SINGLE CELL PROTEIN PRODUCTION WITH LACTOBACILLUS SPECIES AND BACILLUS CLAUSII ON MEDIA CONTAINING SUGARCANE BAGASSE EXTRACT & FISH WASTE HYDROLYSATE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 11, page no.66-71, November-2020, Available :http://www.jetir.org/papers/JETIREI06009.pdf

"SINGLE CELL PROTEIN PRODUCTION WITH LACTOBACILLUS SPECIES AND BACILLUS CLAUSII ON MEDIA CONTAINING SUGARCANE BAGASSE EXTRACT & FISH WASTE HYDROLYSATE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 11, page no. pp66-71, November-2020, Available at : http://www.jetir.org/papers/JETIREI06009.pdf