• Umesh S. Wagh
• Gurunath V. Pandit
• Shailendra V. Dalvi
• Gunesh G. Gundi

Nepafenac is a pro drug with low inherent cyclooxygenase-inhibiting activity. The active non steriodial anti-inflammatory drug compound of nepafenac is called amfenac (2-amino-3-benzoylbenzeneacetic acid) which is an non steriodial anti-inflammatory drug with an arylacetic acid structure exhibiting potent antipyretic and analgesic properties. Nepafenac is designated chemically as 2-amino-3-benzoylbenzeneacetamide with an empirical formula of C15H14N2O2 and molecular weight of 254.28 g/mol. Nepafenac is a member of the new class of non steriodial anti-inflammatory pro drugs for ophthalmic use, providing a novel drug delivery mechanism. The analgesic and anti-inflammatory effect of nepafenac is the result of its fast penetration through the cornea in addition to conversion to amfenac.Present paper describes a high performance thin layer chromatographic for determination of nepafenac from Ophthalmic suspension. Separation was performed on aluminium-backed silica gel 60 F254 HPTLC plates with Toluene : Acetone : Glacial acetic acid (6 : 4 : 0.25) as mobile phase. After development, plates were observed under UV and detection and quantification were performed by densitometry at λ = 254 nm. Detection and quantitation limits for nepafenac is 0.8 µg/mL and 2 µg/mL. Nepafenac response was linear over the range 10 µg/mL to 30 µg/mL. The developed method was validated to determine its accuracy, precision and stability by carrying out linearity and stability experiments. The method developed is simple, fast, accurate and precise and hence can applied for routine quality control analysis from Pharmaceutical preparations / Ophthalmic suspensions.

Nepafenac, HPTLC, Silica Gel, Densitometery, Ophthalmic suspension

"QUANTIFICATION DETERMINATION OF NEPAFENAC IN OPHTHALMIC SUSPENSION USING HIGH PERFORMNACE THIN LAYER CHROMATOGHRAPHY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 6, page no.107-116, June-2023, Available :http://www.jetir.org/papers/JETIRFY06015.pdf

"QUANTIFICATION DETERMINATION OF NEPAFENAC IN OPHTHALMIC SUSPENSION USING HIGH PERFORMNACE THIN LAYER CHROMATOGHRAPHY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 6, page no. pp107-116, June-2023, Available at : http://www.jetir.org/papers/JETIRFY06015.pdf