• Bari V
• kudu M
• Kolekar k
• More N,Yadav N.,Dr.Gupta S
• Arjun P,Sharma A

The ultimate aim of this study is to evaluate the production of protease from Aspergillus Niger under solid state fermentation by utilizing black spotted croaker fish (Ghol fish ) scales powder as a solid substrate Fish scale, the chief waste material of fish processing industries, was enzymatically hydrolysed by protease produced by A. Niger. Trichloroacetic acid buffer was used to maintain the optimum moisture content of the substrate. No separate nutrient sources apart from the fish scales were used .This proves the cost effectiveness and efficiency of these processes. The O.D was taken at 275 nm spectrophotometrically. Solid-state fermentation (SSF) is recognized as an effective method to produce protease. The ultimate aim of this study is to optimize the production of protease from Aspergillus Niger under solid-state fermentation (SSF) by utilizing shrimp shell powder as a solid substrate. It was found that the produced protease from SSF was slightly alkaline. The correlation between factors operating parameters (incubation temperature, inoculums size, moisture content) for enzyme production is analyzed using statistical software.

proteases,A.niger,Proteases application,chymosin,therapeutic agents.

"PRODUCTION AND ANALYSIS OF PROTEASE FROM ASPERGILLUS NIGER USING FISH SCALES AS SUBSTRATE.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 6, page no.170-173, June-2023, Available :http://www.jetir.org/papers/JETIRFY06021.pdf

"PRODUCTION AND ANALYSIS OF PROTEASE FROM ASPERGILLUS NIGER USING FISH SCALES AS SUBSTRATE.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 6, page no. pp170-173, June-2023, Available at : http://www.jetir.org/papers/JETIRFY06021.pdf