• Shiji Thomas

Neem (Azadirachta indica) is considered as one of the most useful medicinal plants that possesses a wide spectrum of biological properties. The present study aims to detect the antibacterial efficacy of various extracts of fresh leaves of Azadirachta indica against different clinical strains. In the present study, biologically active components from the leaves of Azadirachta indica were extracted using four different solvents of increasing polarities (chloroform, methanol, ethanol and water). The antibacterial efficacy of these extracts was determined using agar well diffusion method. Further in this study, qualitative phytochemical screening for identification of various classes of active chemical constituents such as phenols, flavonoids, saponins, terpenoids, glycosides and alkaloids using the methanolic extract. The zones of inhibition observed in the case of gram positive and drug resistant strains were greater compared to the zones of gram-negative ones. The findings of the present antibacterial assay indicates that extract prepared using water exhibited less antibacterial activity compared to other extracts. This study provides scientific understanding to further explore other pharmacological properties of the plant.

Azadirachta indica, antibacterial activity, bacterial strains, phytochemical analysis, HPLC

"PRELIMINARY PHYTOCHEMICAL ANALYSIS AND IN VITRO ANTIBACTERIAL ACTIVITY OF NEEM (AZADIRACHTA INDICA) LEAVES ON BACTERIAL PATHOGENS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.4, Issue 1, page no.209-213, January-2017, Available :http://www.jetir.org/papers/JETIR1701729.pdf

"PRELIMINARY PHYTOCHEMICAL ANALYSIS AND IN VITRO ANTIBACTERIAL ACTIVITY OF NEEM (AZADIRACHTA INDICA) LEAVES ON BACTERIAL PATHOGENS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.4, Issue 1, page no. pp209-213, January-2017, Available at : http://www.jetir.org/papers/JETIR1701729.pdf