UGC Approved Journal no 63975(19)

ISSN: 2349-5162 | ESTD Year : 2014
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Published in:

Volume 2 Issue 7
July-2015
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIR1701753


Registration ID:
321259

Page Number

419-428

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Title

Isolation and Identification of heavy metal resistant bacterial strains from Chavara industrial area, Kollam (Dt) Kerala

Abstract

The occurrence of toxic heavy metals in the environment is geogenic or anthropogenic. In mitigating heavy metal polluted environments several treatment strategies and methods are available such as chemical precipitation, filtration, ion exchange, electrochemical treatment, membrane technologies, adsorption on activated carbon, evaporation, phytoremediation etc. This study involved isolation, identification and molecular characterization of heavy metal and drug resistant bacterial strains from the sediment samples of Vattakayal backwaters, near chavara industrial area. Morphological and biochemical tests were carried out for the identification of seven heavy metal tolerant bacterial isolates. Based on the analysis the isolates were identified as Bacillus cereus, Enterobacter aerogens, Pseudomonas putida, Bacillus licheniformis, Klebsiella pneumonia, Alcaligens faecalis, and Citrobacter kosari. Sequences were matched with previously published bacterial 16S rDNA sequence in the NCBI database using ADVACED BLAST. Antibiotic resistant study showed that 57.14% of the isolates were resistant to at least one antibiotic tested. Phylogenetic tree was constructed by using MEGA4 software and the evolutionary history was inferred by neighbour-joining method. It was found that the strain K1 classified in the branch of Bacillus cereus and K4 in the branch of Bacillus licheniformis.

Key Words

Heavy metal, Bacillus cereus, Bacillus licheniformis, Minimal Inhibitory Concentration

Cite This Article

"Isolation and Identification of heavy metal resistant bacterial strains from Chavara industrial area, Kollam (Dt) Kerala", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.2, Issue 7, page no.419-428, July-2015, Available :http://www.jetir.org/papers/JETIR1701753.pdf

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2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"Isolation and Identification of heavy metal resistant bacterial strains from Chavara industrial area, Kollam (Dt) Kerala", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.2, Issue 7, page no. pp419-428, July-2015, Available at : http://www.jetir.org/papers/JETIR1701753.pdf

Publication Details

Published Paper ID: JETIR1701753
Registration ID: 321259
Published In: Volume 2 | Issue 7 | Year July-2015
DOI (Digital Object Identifier):
Page No: 419-428
Country: Kollam, Kerala, India .
Area: Biological Science
ISSN Number: 2349-5162
Publisher: IJ Publication


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