• Iqbal H Qadri

Almond is one of the Rosaceous stone fruits of temperate crops.There is extreme variability among almond crop plants in terms of fruit size and taste due to cross pollination.The main of this study is to develop an effective protocol for conservation of this Root stock germplasm variability by using embryonal axis’s under invitro regeneration system. Mature tissues of almond plant when used as explants for invitro regeneration are the most recalcitrant among stone fruits.Mature almond embryos from 40 day chilled Kernels after sterilization were cultured onMS (1/2) medium under the influence of BAP (0.5,2.0,2.5 and 3.0 µM ) and IBA(1.0,2.5 and 5.0 µM) in presence or absence of GA3(0.5 µM).After 8 weeks of culture period the results were evaluated. Adventitious shoot regeneration was observed at BAP (2.0,2.5 and 3.0 µM ) and IBA(1.0 µM) combination. Highest regeneration (62.22%) with average shoot number of 8 per explant was observed at BAP (2.5 µM ) and IBA(1.0 µM).Addition of GA3 (0.5 µM) significantlydecreased the regeneration frequency (51.11%), with average shoot number of 2 per explant, at this concentration. In another study embryos from immature nuts without chilling period were cultured on BAP (2.5,5.0,7.5 and 10.0 µM ) and IBA(2.5 and 10.0 µM) combination and after 8 weeks of culture period adventurous shoot regeneration was observed at BAP (5.0,7.5 and 10.0 µM ) and IBA(2.5 and 5.0 µM) combination. Highest adventitious shoot regeneration (55%) with average shoot number of 12 per explant was recorded at BAP (7.5 µM ) and IBA(2.5 µM) Combination.

Almond; embryo; regeneration

"Adventitous Shoot Regeneration From Mature And Immature Embryo Of Paper Shelled Almond Rootstock Cv. Parbhat ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.4, Issue 6, page no.347-357, June-2017, Available :http://www.jetir.org/papers/JETIR1706071.pdf

"Adventitous Shoot Regeneration From Mature And Immature Embryo Of Paper Shelled Almond Rootstock Cv. Parbhat ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.4, Issue 6, page no. pp347-357, June-2017, Available at : http://www.jetir.org/papers/JETIR1706071.pdf