• Purnima Sharma

The textile industry not only plays a crucial role in our everyday lives, but it is also a major factor in developing the global economy. One of the environmental issues is the disposal of large amounts of radioactive dyes into the water, leading to significant environmental contamination. Bacillus subtilis , a bacteria with biodegradation property found in Punjab water bodies that decolorize the industrial waste water because bacteria contain laccase enzyme that is multi copper protein that can oxidize inorganic and aromatic deys such as azo dyes , syringaldazine and 2, 6-dimethoxyphenol. Bacteria is more pronounced than fungus because it contain spore coat A type protein that help them to survive in unfavourable conditions such as high temperature and ph. Our present study deals with Characterisation of Bacillus subtillus bacteria containing Spore coat protein (Uniprot id : M9Y1F2, Gene id: cotA ),textile dye degrading enzymes laccase, through analyzing their structural and functional properties using standard computational tools. The spores were used for bleaching RBBR, alizarin, Congo red, methyl orange, and methyl violet. The bleaching rate was 90% in the treatment of RBBR and alizarin red. Physico-chemical characterization confirmed acidic and hydrophilic nature of both laccase enzyme . Higher aliphatic index ascertained the thermostability of laccase. Negative GRAVY value of the laccase confirmed better water interaction of the enzyme. Functional Analysis revealed that bacteria contain spore coat protein with oxidoreductase activity, with three functional domains Cu-oxidase (PF00394), , Cu-oxidase_2(PF07731), Cu-oxidase_3(PF07732) that help in survive under harsh conditions. Laccase has high substrate selectivity and more effective in dye decolorization. To know the bacterial laccase effect on dye by docking, MGL Tool and AutoDock suit are used.

Dye decolorization, Laccase, Textile dyes, Microbial bioremediation.

"Computational Analysis of Textile Dyes Decolorizing Bacteria Found in Industrial Waste Water", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.4, Issue 8, page no.515-532, August-2017, Available :http://www.jetir.org/papers/JETIR1708082.pdf

"Computational Analysis of Textile Dyes Decolorizing Bacteria Found in Industrial Waste Water", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.4, Issue 8, page no. pp515-532, August-2017, Available at : http://www.jetir.org/papers/JETIR1708082.pdf