• Dr. Ashok Nagar
• Dr. Ashwani Kumar Verma
• Laxmi Meena

Pea (Pisum sativum L.) is an important leguminous seed crop grown for vegetable and food purpose. Leaf spot disease of pea caused by Xanthomonas campestris pv. pisi is a threat for the production, yield and quality of seeds. During field surveys, pea seeds were collected from major pea growing districts of Rajasthan. The collected seeds upon incubation yielded bacterial pathogen identified as Xanthomonas campestris pv. pisi by biochemical and molecular characterization using 16s rRNA sequencing. The pathogen was detected in one hundred and nineteen seed samples on YDC agar medium with 3-89.5% incidence from different districts of Rajasthan. To study the phytopathological effects and seed to seedling disease transmission three different methods viz. Petri plate method, water agar test tube symptom test and pot experiment were used. In Petri plate method and test tube seedling symptom test, the pathogen observed as white to pale cream oozing on and around the seeds. The pathogen caused the symptoms initiated as irregular brown to black spots of radical and plumule which later showed rotting. Symptomatic seedlings showed bacterial oozing, brown irregular water-soaked spots and browning of cotyledonary leaves which later showed blighted collapsed hypocotyls. Loss of germination and mortality of seedlings were found higher in shrivelled discoloured seeds. The pre- and post emergence losses recorded due to pathogens studied.

Pea seeds, Xanthomonas campestris pv. Pis, Seedling symptoms, transmission

"Incidence, Phytopathological effects and disease transmission of leaf spot disease of pea in Rajasthan, India", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.4, Issue 11, page no.1417-1419, November-2017, Available :http://www.jetir.org/papers/JETIR1711220.pdf

"Incidence, Phytopathological effects and disease transmission of leaf spot disease of pea in Rajasthan, India", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.4, Issue 11, page no. pp1417-1419, November-2017, Available at : http://www.jetir.org/papers/JETIR1711220.pdf