UGC Approved Journal no 63975(19)

ISSN: 2349-5162 | ESTD Year : 2014
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Published in:

Volume 5 Issue 3
March-2018
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIR1803097


Registration ID:
180645

Page Number

516-520

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Title

PLANTLET REGENERATION FROM EMBRYONAL SHOOT EXPLANTS OF PINUS ROXBURGHII SARG.

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Abstract

Abstract - Embryonal shoot explants from in vitro raised seedlings of Pinus roxburghii were cultured on MS(x1/2) basal medium fortified with various cytokinin concentrations. Explants produced green friable callus at basal ends of explant on MS(x1/2) medium enriched with BAP(2.5µM).However other BAP concentrations(5,10,15µM) favoured multiple shoot regeneration. With BAP(5µM) maximum number of shoots(10±0.5) was recorded. In other trials when BAP was replaced by Kn(2.5 5,10,15 ,20 ,25µM) only calluas induction was observed at the basal ends of explants. The cultures with multiple microshoots were subcultured on basal medium for shoot elongation. Isolated shoots produced rooting response on MS(x1/2) basal medium after 8-10 weeks of culture period. Rooted plantlets were transferred to green house for hardening and field trials.

Key Words

Keywords- Embryonal shoot,Pinus roxburghii, Explant, multiple shoot, plantlet, MS medium: Murashige and Skoog (1962).

Cite This Article

"PLANTLET REGENERATION FROM EMBRYONAL SHOOT EXPLANTS OF PINUS ROXBURGHII SARG.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 3, page no.516-520, March-2018, Available :http://www.jetir.org/papers/JETIR1803097.pdf

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2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"PLANTLET REGENERATION FROM EMBRYONAL SHOOT EXPLANTS OF PINUS ROXBURGHII SARG.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 3, page no. pp516-520, March-2018, Available at : http://www.jetir.org/papers/JETIR1803097.pdf

Publication Details

Published Paper ID: JETIR1803097
Registration ID: 180645
Published In: Volume 5 | Issue 3 | Year March-2018
DOI (Digital Object Identifier):
Page No: 516-520
Country: Srinagar, Jammu & Kashmir, India .
Area: Biological Science
ISSN Number: 2349-5162
Publisher: IJ Publication


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