• Ameerjani Shaik
• Yousuf Anwar Syed

One of the most emotionally and economically destructive burdens on the face of globe is HIV infection. Mankind is still helpless due to non availability of a safe vaccine and non toxic therapy. There is an urgent need for a non toxic, non expensive, eco friendly possibly a plant derived drug which will block any one or many or all the stages of life cycle of HIV. Traditional knowledge is valid and necessary and awaits its currently relevant wider application for human benefit. According to Indian Traditional Knowledge, nine plants represent nine astronomical entities. Nine plants were identified and authenticated. Powdered explants of nine plants were macerated individually, separately using ethanol and chloroform & evaporated. From our other experiments GCMS analysis of individual extracts was done to identify compounds and anti-HIVactivity of the individual compounds if any recorded, was known from chemdb NIAID database. The crude extracts of certain selected individual plants were mixed together forming biherabal, triherbal, tetraherbal and pentaherbal extract samples. Also, all nine different powdered explants were mixed together forming a unique polyherbal sample which was also macerated separately with ethanol and chloroform and evaporated to yield ethanol derived crude polyherbal extract and chloroform derived crude polyherbal extract which were mixed together to form an unique double polyherbal extract sample. Extract samples were tested against HIV-1-RTp66 using assay kit (Immunodiagnostics, USA). All the three biherbal, one tri, one tetra and one penta herbal extract sample showed more than 50% of inhibition at different concentration of HIV-1-RT rp66. A biherbal extract sample made of stem of Ficus religiosa (Linn.) and leaves of Desmostachya bipinnata (Linn.) Stapf. exhibited maximum of 88.88 % of inhibition. Double polyherbal extract sample showed maximum of 70.58% of inhibition. Further in-vitro research on these extract samples will be beneficial.

Indian Traditional Knowledge, Nine plant forest, Nine plants, Polyherbal samples, Maceration, anti-HIV-1-RT p66

"In-vitro anti HIV activity of plants of nine-planet forest", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 7, page no.297-303, July-2018, Available :http://www.jetir.org/papers/JETIR1807200.pdf

"In-vitro anti HIV activity of plants of nine-planet forest", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 7, page no. pp297-303, July-2018, Available at : http://www.jetir.org/papers/JETIR1807200.pdf