• P.PUNITHARAJ
• S. VENKATESAN
• M. KANNAN
• G. MANIVANNAN
• J. MUGIL

Abstract: This work targets on β-glucan synthesis and analysis of mycochemicals present in the mushroom, Lycoperdon perlatum. The samples were collected from coastal region of southern Tamilnadu. The sample, that the surface sterilized fruit body was dried and stored in powder form. The mycochemicals were examined from the mushroom fruit body dried powder. The mushroom mycelium was grown on PDA plate by the spore inoculation. The production of extracellular enzymes from mushroom mycelium was detected. Beta-glucan was isolated from mycelium using submerged fermentation technique and purified. It was acid hydrolyzed using tri-fluoro acetic acid (TFA) and analyzed by thin-layer chromatography. Total protein content present in the mushroom was measured by Lowry's method. As like total carbohydrate content was measured by phenol and sulfuric acid method that contain 56% crude β-glucan. The result obtained from this study has shown the potential of Lycoperdon perlatum mushroom extract as a potent therapeutic agent and a food supplement.

Lycoperdon perlatum, Beta-glucan, Mycochemicals, Extra cellular enzymes

"ΒETA GLUCAN SYNTHESIS AND MYCOCHEMICALS SCREENING OF LYCOPERDON PERLATUM COLLECTED FROM SAYALGUDI, SOUTHERN TAMILNADU, INDIA", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 7, page no.441-447, July-2018, Available :http://www.jetir.org/papers/JETIR1807785.pdf

"ΒETA GLUCAN SYNTHESIS AND MYCOCHEMICALS SCREENING OF LYCOPERDON PERLATUM COLLECTED FROM SAYALGUDI, SOUTHERN TAMILNADU, INDIA", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 7, page no. pp441-447, July-2018, Available at : http://www.jetir.org/papers/JETIR1807785.pdf