• Tonmoy Sarkar
• Arindam Chakraborty
• Kiron Bhakat
• Ekramul Islam

In the present study a soil bacterium was isolated from potato rhizosphere and characterized in terms of morphology, biochemistry and phylogeny. This bacterium showed antagonistic behavior to other soil bacterium and type strains. DNA from the pure culture was isolated and used in PCR amplification of 16S rRNA gene followed by DNA sequencing and phylogenetic analysis. Phenotypical, biochemical and 16S rRNA gene-based phylogenetic analysis revealed its closer affiliation to the genus Bacillus but it did not share any clad with type strains and hence representing a new novel lineage of Bacillus and named as Bacillus sp. EIKU1. Bacillus sp. EIKU1 showed antagonistic behaviour mostly against Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus. This study paves the way for further characterisation of Bacillus sp. EIKU1 for its optimum utilisation as a source of antimicrobials.

Rhizosphere; Bacillus; Antagonistic behaviour; Phylogeny

"PHYLOGENETIC CHARACTERIZATION OF A SOIL BACTERIUM SHOWING ANTAGONISTIC BEHAVIOUR", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 8, page no.548-551, August-2018, Available :http://www.jetir.org/papers/JETIR1807805.pdf

"PHYLOGENETIC CHARACTERIZATION OF A SOIL BACTERIUM SHOWING ANTAGONISTIC BEHAVIOUR", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 8, page no. pp548-551, August-2018, Available at : http://www.jetir.org/papers/JETIR1807805.pdf