UGC Approved Journal no 63975

ISSN: 2349-5162 | ESTD Year : 2014
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Published in:

Volume 5 Issue 7
July-2018
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIR1807943


Registration ID:
185551

Page Number

320-323

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Title

SPECIFICITY OF MYCORRHIZAL FUNGI ISOLATED FROM THE ROOTS OF VANDA TESTACEA FROM FIVE DIFFERENT REGIONS USING DNA BARCODING

Abstract

Orchidaceae is one of the longest families of flowering plants which consist of nearly 25000 species. Vanda testacea is an epiphytic perennial which is occurring from the Indian subcontinent to Indochina at the elevations of 500 to 2000 meters. Mycorrhizal association of orchids is essential because for its seed germination and seedling development. The present study is carried out to identify the specific mycorrhizal fungi from the roots of Vanda testacea from five different locations using DNA barcoding. Two loci used for this purpose were Internal Transcribed Spacer region and Large Subunit region. The new DNA sequences were identified using BLAST software tool (NCBI, USA) and deposited in NCBI. The DNA sequences that shown maximum similarity to query sequences were selected and analysed using MEGA 6.0 (Molecular Evolutionary Genetics Analysis) for phylogenetic analysis. The phylogenetic tree was generated based on Neighbour joining method. It shows the similarity in the mycorrhizal fungi identified from five different locations of Vanda testacea.

Key Words

Vanda testacea, Mycorrhizal Specificity, Phylogeny, Tulasnella species, DNA barcoding

Cite This Article

"SPECIFICITY OF MYCORRHIZAL FUNGI ISOLATED FROM THE ROOTS OF VANDA TESTACEA FROM FIVE DIFFERENT REGIONS USING DNA BARCODING", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 7, page no.320-323, July-2018, Available :http://www.jetir.org/papers/JETIR1807943.pdf

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2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"SPECIFICITY OF MYCORRHIZAL FUNGI ISOLATED FROM THE ROOTS OF VANDA TESTACEA FROM FIVE DIFFERENT REGIONS USING DNA BARCODING", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 7, page no. pp320-323, July-2018, Available at : http://www.jetir.org/papers/JETIR1807943.pdf

Publication Details

Published Paper ID: JETIR1807943
Registration ID: 185551
Published In: Volume 5 | Issue 7 | Year July-2018
DOI (Digital Object Identifier):
Page No: 320-323
Country: trichy, tamilnadu, India .
Area: Biological Science
ISSN Number: 2349-5162


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