• Mrinalini C. Damle
• Antra Sen

The objective of the study was to develop and validate a simple, specific, precise and accurate HPLC method for the estimation of Canagliflozin in bulk and tablet dosage form. Chromatographic separation was achieved by using Acetonitrile: Water (pH adjusted to 4.5 using 0.1% v/v Ortho -phosphoric acid) as mobile phase in a ratio of 50:50 v/v. Chromatographic separation was achieved using Neosphere C18 column (150×4.6mm i.d. 3.5µm) at 1ml/min as flow rate. The detection was carried out at 291nm using PDA detector. The retention time was observed to be 4.7 ±0.05min. This drug was subjected to various stress degradation conditions as per ICH Q1A (R2). Linearity was found to be in the concentration range of 10-50µg/ml with R2 =0.996 .The suitability of this HPLC method for quantitative estimation of Canagliflozin was proved by validation as per ICH Q2A(R1) guidelines and can be used for the routine analysis of Canagliflozin in bulk and tablet dosage form.

Canagliflozin, HPLC, stress degradation, Stability indicating

"Development and validation of a stability-indicating HPLC method for the estimation of Canagliflozin", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 8, page no.213-220, August-2018, Available :http://www.jetir.org/papers/JETIR1808182.pdf

"Development and validation of a stability-indicating HPLC method for the estimation of Canagliflozin", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 8, page no. pp213-220, August-2018, Available at : http://www.jetir.org/papers/JETIR1808182.pdf