• Alabi Titilayo Latifat
• Pankaj Kumar Teli
• Aarti Bisht
• Vinod Kumar Gupta

Plants have the ability to synthesize a wide variety of chemical compounds that are used to perform important biological functions, and to defend against attack from predators such as insects, fungi and herbivorous animals.In traditional medicine, plants have been used to prevent and cure infectious conditions and diseases caused by pathogenic microorganism.Therefore this study aimed to investigate thecombined effect of Eucalyptus grandis, Moringa oleifera, Punica granatum and Syzygium aromaticum crude plant extracts against pathogenic organisms and using the extract with the highest synergistic or additive effect against the selected bacteria strains to formulate sanitizer as a preventive measure against the spread of disease resulting from these bacteria.This study was conducted against four bacteria strains, Klebsiella pneumonia, Proteus mirabilis, Staphylococcus aureus and Staphylococcus epidermidis using disc diffusion method. The interaction between the two and three combination extracts revealed that they were synergistic, additive and antagonistic against the four selected strains of bacteria. The highest additive effect was observed in KP and PM when there was a combination of PCE while a synergistic effect was observed in KP when there was a combination of Po & Mo. Staphylococcus epidermidis showed significant inhibitory effect to all the single extract used but displayed antagonistic effect to all the two and three combination extracts. Combination of Eu&Cl and the single extract of pomegranate showed indifferent effects against Staphylococcus aureus. The formulated sanitizers, however, were effective against the hand microbiome in volunteer samples with no side effects on human tissue.

Phytochemical analysis, Eucalyptus grandis, Moringa oleifera, Punica granatum, Syzygium aromaticum

"ANTIBACTERIAL ACTIVITIES OF THE COMBINED EXTRACTS OF Eucalyptus grandis, Moringa oleifera, Punica granatum and Syzgium aromaticum AGAINST PATHOGENIC ORGANISMS AND FORMULATION OF SANITIZER ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 9, page no.185-193, September-2018, Available :http://www.jetir.org/papers/JETIR1809333.pdf

"ANTIBACTERIAL ACTIVITIES OF THE COMBINED EXTRACTS OF Eucalyptus grandis, Moringa oleifera, Punica granatum and Syzgium aromaticum AGAINST PATHOGENIC ORGANISMS AND FORMULATION OF SANITIZER ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 9, page no. pp185-193, September-2018, Available at : http://www.jetir.org/papers/JETIR1809333.pdf