• Dr. B. Vishnupriya
• Dr. K. Selvam

This study was focused on partial purification and characterization of keratinase from Streptomyces albogriseolous NGP 2, isolated from marine sediment of south Indian coastal region. In purification steps, 1.56 fold purification was achieved after 85% ammonium precipitation of the with 8.03% recovery. In further purification steps, 8.34 fold purified keratinase was recovered by Sephadex G-100 chromatography with 1.79% of recovery. The specific activity of purified enzyme was 1045.83 U/mg. Zymogram of crude enzyme on native-PAGE presented bands with keratinase activity of molecular weight and Isoelectric point were 40 kDa and 8.3. These findings suggested that the keratinase may have much industrial applications.

Marine sediment, Molecular weight, Isoelectric point, Zymogram

"Kinetic studies of partially purified keratinase from Streptomyces albogriseolous NGP 2", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 9, page no.297-304, September-2018, Available :http://www.jetir.org/papers/JETIR1809448.pdf

"Kinetic studies of partially purified keratinase from Streptomyces albogriseolous NGP 2", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 9, page no. pp297-304, September-2018, Available at : http://www.jetir.org/papers/JETIR1809448.pdf