• Dr. M. Subba Rao
• Gopinath. K

A validated stability-indicating RP-HPLC method for benzydamine HCl and Chlorhexidine gluconate was developed by separating its degradation products on a C18 (150x4.6mm, 3.5µm) Waters symmetry column using 0.1% phosphoric acid in water and acetonitrile in simple gradient at a flow rate 1.0 ml/min. The column effluents were monitored by a photodiode array detector set at 230nm. The method was validated in terms of specificity, linearity, accuracy, precision, detection limit, quantification limit and robustness. Forced degradation of Benzydamine HCl and Chlorhexidine gluconate was carried out under acidic, basic, peroxide, reduction, thermal, photo and hydrolysis conditions. The proposed method is validated as per ICH Q2 (R1) guidelines

Benzydamine HCl, Chlorhexidine gluconate, RP-HPLC

"DEVELOPMENT AND VALIDATION OF A STABILITY INDICATING RELATED SUBSTANCES OF BENZYDAMINE HYDROCHLORIDE AND CHLORHEXIDINE GLUCONATE BY RP-HPLC AND ITS DEGRADATION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 10, page no.548-556, October-2018, Available :http://www.jetir.org/papers/JETIR1810183.pdf

"DEVELOPMENT AND VALIDATION OF A STABILITY INDICATING RELATED SUBSTANCES OF BENZYDAMINE HYDROCHLORIDE AND CHLORHEXIDINE GLUCONATE BY RP-HPLC AND ITS DEGRADATION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 10, page no. pp548-556, October-2018, Available at : http://www.jetir.org/papers/JETIR1810183.pdf