• Pulak Das
• Indrani Chandra

Pyk10 is a root and hypocotyl specific myrosinase from Arabidopsis thaliana. Myrosinase are a group of isoenzymes catalyzing the hydrolysis of glucosinolates. pyk10 is a root and hypocotyls specific myrosinase gene from Arabidopsis thaliana. In order to study the pyk10 promoter and the genomic structure of the gene, The promoter fragment of pyk10 was cloned by PCR method from Arabidopsis thaliana genomic DNA. Nucleotide sequence analysis showed that the fragment cloned was consist of 797bp. Comparison the sequence obtained with that reported (GenBank Accession no. AJ292756, CP002686, AC011436) showed that the nucleotide homology was as high as 100%. The pyk10 promoter contains regulatory sequence elements that have shown to be functional cis-sequences in other plant promoters, such as ACGT-, CANNTG-, GATA and I-box-cis-elements, which are binding sites for organ and tissue-specific transcription factors.

PYK 10,PCR-Polymerase Chain Reaction, cloning,promoter, Arabidopsis thaliana

"ISOLATION, MOLECULAR CLONING AND CHARACTERIZATION OF PYK10 PROMOTER REGION FROM ARABIDOPSIS THALIANA", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 11, page no.461-464, November-2018, Available :http://www.jetir.org/papers/JETIR1811261.pdf

"ISOLATION, MOLECULAR CLONING AND CHARACTERIZATION OF PYK10 PROMOTER REGION FROM ARABIDOPSIS THALIANA", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 11, page no. pp461-464, November-2018, Available at : http://www.jetir.org/papers/JETIR1811261.pdf