• Edwin Chelliah D
• Ranjitsingh A. J. A
• Kalirajan K
• Athinarayanan G

Haemophilus influenzae type b (Hib) causes many severe diseases, including epiglottitis, pneumonia, sepsis, and meningitis (David W McCormick and Elizabeth M Molyneux, 2011). In developed countries, the annual incidence of meningitis caused by bacteria is approximately 5–10 cases per population of 100,000. The Hib conjugate vaccine is considered protective, safe and Effective control (Verma R et al., 2011). Many factors may play a role in the immunogenicity of Hib conjugate vaccines, such as the polysaccharides and proteins carrier used in vaccine construction, as well as the method of conjugation (Heikki Peltola, 2000). A Hib conjugate vaccine has been constructed via chemical synthesis of a Hib saccharide antigen. Two models of carbohydrate-protein conjugate have been established, the single ended model (terminal amination-single method) and cross-linked lattice matrix (dual amination method). HPAEC-PAD (high performance anion-exchange chromatography with pulsed amperometric detection) method was used for free polysaccharide estimation using PRP standard obtained from NIBSC. Maintain the PRP content in different stages are more vital for the effective production of Hib conjugate vaccine.

Haemophilus type b conjugate, Polyribosyl ribitol phosphate (PRP), immunogenicity.

"Upholding the Polyribosyl ribitol phosphate (PRP) content in different stages in manufacturing of Haemophilus influenzae type b (Hib) conjugate vaccine", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 11, page no.625-634, November-2018, Available :http://www.jetir.org/papers/JETIR1811282.pdf

"Upholding the Polyribosyl ribitol phosphate (PRP) content in different stages in manufacturing of Haemophilus influenzae type b (Hib) conjugate vaccine", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 11, page no. pp625-634, November-2018, Available at : http://www.jetir.org/papers/JETIR1811282.pdf