• Sheeja, H. M
• Vinoliya Josephine Mary , J
• Mary Mettilda Bai, S

Natural agglutinins with diverse specificity for diverse mammalian erythrocytes were identified in different tissues of sea urchin Stomopneustes variolaris. Of the various tissues analyzed for the distribution of agglutinin, maximum HA was observed in the coelomic fluid of the sea urchin S. variolaris. Physico-chemical properties of the coelomic fluid agglutinin showed that it specifically depends on Ca2+ for its activity, stable to wide range of pH and sensitive to temperature and EDTA. Disappearance of agglutinability following cross adsorption revealed the presence of a single agglutinin. The coelomic fluid agglutinin was inhibited by sugars such as GalNAc and GlcNAc and was effectively inhibited by the glycoprotein PSM. BSM, transferrin, α-acid glycoprotein and also animal body fluids such as saliva, milk and urine inhibited the hemagglutination of the coelomic fluid.

Agglutinin, Coelomic fluid, lectin

"Identification and physico-chemical characterization of a natural agglutinin from coelomic fluid of the sea urchin Stomopneustes variolaris (Lamarck)", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 11, page no.671-680, November-2018, Available :http://www.jetir.org/papers/JETIR1811789.pdf

"Identification and physico-chemical characterization of a natural agglutinin from coelomic fluid of the sea urchin Stomopneustes variolaris (Lamarck)", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 11, page no. pp671-680, November-2018, Available at : http://www.jetir.org/papers/JETIR1811789.pdf