• Sonu Bhatia
• Jagtar Singh
• Jagtar Singh

α-Galactosidase hydrolyze 1,6-linked-α-galactose residues from raffinose, stachyose, and melibiose. Bacteria show great potential for production of α-galactosidases due to elevated expression levels, extracellular secretion and scope for improvement of yield by optimization of culture conditions. Thermostable α-galactosidase has been obtained from extremophilic bacteria can withstand the high-processing temperatures employed in industries. Biochemical characterization of the enzyme is important for their successful utilization in numerous biotechnological applications. In the present study, Manikaran hot springs (Parvati valley, Kullu district, Himachal Pradesh, India) were selected to screen bacterial strains producing α-galactosidase followed by its characterization. Culture dependent approach was used to screen 39 α-galactosidase positive isolates using X-gal plate assay. Morphological, microscopic and biochemical characterization was performed on positive isolates.

α-Galactosidase, hot springs, thermophiles, bacterial diversity

"SCREENING AND CHARACTERIZATION OF α-GALACTOSIDASE PRODUCING BACTERIAL STRAINS ISOLATED FROM INDIAN HOT SPRING", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 11, page no.105-114, November-2018, Available :http://www.jetir.org/papers/JETIR1811C63.pdf

"SCREENING AND CHARACTERIZATION OF α-GALACTOSIDASE PRODUCING BACTERIAL STRAINS ISOLATED FROM INDIAN HOT SPRING", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 11, page no. pp105-114, November-2018, Available at : http://www.jetir.org/papers/JETIR1811C63.pdf