• Hemlata M. Nimje
• T. V. Chorage
• Mr. S. V. Mirje
• Dr. S. B. Wankhede

A simple, accurate, precise, robust and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of orlistat in the capsules formulation. Orlistat was chromatographed on silica gel 60 F254 TLC plate, as a stationary phase. The mobile phase was toluene: ethyl acetate: phosphopolymbdic acid (6:4:0.3 v/v/v) which gave a dense and compact spot of orlistat with a Rf value of 0.39±0.03. The quantification was carried out at 581.00 nm. The method was validated in terms of linearity, accuracy, precision, robustness and specificity. To justify the suitability, accuracy and precision of the proposed method, recovery studies were performed at three different concentration levels. Statistical analysis proved that the proposed method is accurate and reproducible with linearity in the range of 1000 to 6000 ng/spot. The limit of detection and limit of quantification for orlistat were 25 and 78 ng/spot, respectively. The proposed method can be employed for the routine analysis of orlistat as well as in pharmaceutical formulations.

HPTLC densitometry, orlistat, capsules, validation

"DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR ESTIMATION OF ORLISTAT AND ITS FORMULATIONS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 12, page no.32-43, December-2018, Available :http://www.jetir.org/papers/JETIR1812006.pdf

"DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR ESTIMATION OF ORLISTAT AND ITS FORMULATIONS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp32-43, December-2018, Available at : http://www.jetir.org/papers/JETIR1812006.pdf