• Nita Thapa
• Janardan Lamichhane
• Meena Maiya Suwal
• Sudina Bhuju
• Dhhurva P. Gauchan

In vitro propagation of Bambusa balcooa Roxb. has been studied which is considered as one of the most significant commercial bamboo species. MS (Murashige and Skoog) basal media supplemented with 5mg/l BAP (6-Benzylaminopurine), 0.8% agar, 3% sucrose and 100mg/l inositol was found to be most suitable for bud break. Maximum number of shoots, 12.67±2.08 was observed after first subculture and 95.67±12.89 after second subculture in MS media which was supplemented with 3mg/l BAP. Subculture was then performed regularly within 3 weeks of time period so as to prevent browning of shoots in media. Optimal rooting was observed on MS media supplemented with 4.5mg/l NAA (Naphthalene acetic acid) with no supply of cytokinin. Maximum number of roots obtained was 236±11.53 with average root length of 13±0.5 after 45-50 days of culture in rooting media. No response was observed when shoot propagules were transferred to rooting media supplemented with IAA (Indole-3-acetic acid) and IBA (Indole-3-butyric acid). Response of explant to different sucrose concentration was also studied where 3% sucrose was the most suitable one where maximum shoot number was 3.33±1.15 with number of leaves 3.66±1.15. 80% success in hardening process was achieved when transferred to greenhouse. During acclimatization, 90% success was achieved when transferred to polybag containing 1:1:2 ratios of soil: ash: vermicompost whereas 70% success in 1:1:2 ratios of soil: sand: vermicompost. Invitro raised plantlets were thus successfully hardened and acclimatized as well. Large scale propagation of B. balcooa species can be regenerated in a time span of 6-7 months which can be time saving as well as economically useful.

Bambusa balcooa, micropropagation, axillary bud, explant

"Invitro assessment of Bambusa balcooa Roxb. for micropropagation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 12, page no.464-469, December-2018, Available :http://www.jetir.org/papers/JETIR1812062.pdf

"Invitro assessment of Bambusa balcooa Roxb. for micropropagation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp464-469, December-2018, Available at : http://www.jetir.org/papers/JETIR1812062.pdf