• Rahul Wankhede
• Sadhana Sathaye

Enzymes bind with chemical reactants called substrates. There may be one or more substrates for each type of enzyme, depending on the particular chemical reaction. This research is based on this enzyme-substrate interaction for the microorganism. For the specific substrate, the organisms will secrete the specific enzyme to digest that substrate. Lactose as a substrate is taken for the production of lactase by the E.coli. In this research, production of lactase (in situ) from the E.Coli is optimized. Temperature, substrate concentration, time and pH are taken as the production parameters for the lactase production. The isolation of the lactase is carried out by the aqueous two phase system (ATPS). PEG 4000, anhydrous potassium phosphate and water is used for the preparation of ATPS. Lactase enzyme activity was measured by the rate of hydrolysis of o-nitrophenyl -D-pyranogalactoside. The efforts to tackle these diseases like lactase and sucrase-isomaltase deficiency, glucose-galactose malabsorption, galactosaemin, hereditary fructose intolerance and the glycogen storage diseases will be further strengthened.

lactase, -galactosidase, E.coli, aqueous two phase system.

"Production of lactase from microorganism and purification of it.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 12, page no.609-624, December-2018, Available :http://www.jetir.org/papers/JETIR1812085.pdf

"Production of lactase from microorganism and purification of it.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp609-624, December-2018, Available at : http://www.jetir.org/papers/JETIR1812085.pdf