• REX RICHARD DSOUZA
• Dr. Nayna Chandak

ABSTRACT Cellulose holds the reputation of the most abundant renewable biomass on the earth. Ethanol produced from cellulosic biomass is one of the most promising renewable fuel source replacing fossil fuels With the advantage of zero net contribution of carbon dioxide to the atmosphere. Hence, the bioconversion of cellulose into ethanol plays an important role for sustainable development .micro-organism having the great ability to degrade cellulosic compounds is of great importance from different ecological point of view. The present study focuses on the isolation and identification of cellulose degraders from sources like Grave Yard Soil And Termite Tubes aims at isolation and characterization of cellulose degrading bacterial fruit and vegetable decaying waste such a sugarcane bagasse, rice hay, tadgola fruit, grass, cellulose degraders were isolated and identified and simultaneously the effect of different environmental parameters such as Ph (5-8), Temperature (30-45), Substrate concentration (0.2%-1.5%), and incubation period (48hours-120 hours) were studied for optimization of growth. Enzyme production was carried out from the isolated degraders. The enzyme was partially purified by Ammonium sulphate precipitation and dialysis. Enzyme assay was carried by dinitrosalycylic acid (DNSA) method to confirm production of cellulose. There for current study mainly focuses on isolation, optimization and identification of cellulose-degraders from grave yard soil and termite tubes selected bacterial isolates were co-cultured with Saccharomyces cerevisiae for simultaneous saccharification and fermentation. Ethanol production was positively tested after five days of incubation with acidified potassium dichromate. Our results suggested that enzyme secreted by this bacteria strain could be a potential source of cellulase enzyme for the development of hydrolysis cellulase for cellulosic biomass and for a further aid of bioconversion of ethanol. Keywords: Cellulose–degraders.Cellulose, optimization, bio-ethanol production, renewable fuel source.  

"To Isolate And Identify Cellulose Degrading Bacteria From Grave Yard Soil And Termite Tubes And ITS Applications", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 12, page no.632-646, December-2018, Available :http://www.jetir.org/papers/JETIR1812183.pdf

"To Isolate And Identify Cellulose Degrading Bacteria From Grave Yard Soil And Termite Tubes And ITS Applications", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp632-646, December-2018, Available at : http://www.jetir.org/papers/JETIR1812183.pdf