• PRASAD GAMPALA
• praveena bomidi

Amino acids are the basic bioelements of proteins, which are the most important macromolecules for the functions of humans and animals. Out of the 20 L-amino acids, ecumenically found in most of living organisms, L-lysine is one of the 9 amino acids which are essential for human and animal nutrition. L-lysine is useful as medicament, chemical agent, food material (food industry) and feed additive (animal food). Its demand has been steadily increasing in recent years and several hundred thousands tones of L-lysine (about 800,000 tones/year) are annually produced worldwide almost by microbial fermentation. The objective of this project is to study on L-lysine production by variation of process parameters (temperature and rpm) and variation of fermentation medium composition ( carbon and nitrogen source) and effect of biotin by Corneybacterium glutamicum in shake flask bioreactor. Stirrer speed, temperature and inoculums medium compositions are varying parameters in Stirred tank bioreactor. . The estimation of Lysine takes places by using Ninhydrin method.

: Lysine, corneybacterium glutamicum, biotin,glucose,ammoniumsulphate.

"STUDIES OF VARIOUS PARAMETERS ON L-LYSINE PRODUCTION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 12, page no.44-50, December-2018, Available :http://www.jetir.org/papers/JETIR1812308.pdf

"STUDIES OF VARIOUS PARAMETERS ON L-LYSINE PRODUCTION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp44-50, December-2018, Available at : http://www.jetir.org/papers/JETIR1812308.pdf