• Dr Sona S Dev
• Sneha Francis
• Vineetha K.U

Abstract - Brinjal or egg plant is an agronomically and economically important solanaceous crop. It is rich in nutrients and is very much beneficial to human health. Brinjal is prone to infection by fungal, bacterial and viral pathogens as well as insect pests leading to significant economic loss. Some of the diseases that affect brinjal are fruit rot, Verticillium wilt, bacterial wilt etc. In such conditions understanding the molecular basis of disease, pest and herbicide resistance in plants proves to be helpful. The identification of resistance gene analogs holds great promise to minimize the extensive use of fungicides and to develop disease resistant plants. In the present study, degenerate primers based on conserved regions of NBS-LRR were used to amplify and clone Resistant Gene Analogs (RGAs) from cultivated (Solanum melongena) / wild species (Solanum surattense, Solanum torvum) of brinjal. Sequencing of the cloned gene were carried out. Comparison of their predicted amino acid sequences with each other and other amino acid sequences of known R-genes revealed high level of identity with NBS-LRR family of RGAs deposited in GenBank. A phylogenetic tree constructed incorporating the sequences of the closest type of RGAs, using MEGA 6.0 software revealed genetic divergence among the three isolated RGAs. S melongena and S.torvum were closely related, while S surattense was in another sub-cluster. The NBS analogs that we isolated can be used as guideline to eventually isolate numerous R-genes in brinjal.

Key words - NBS-LRR, Solanum sp, Resistance Gene Analogs (RGAs)

"MOLECULAR CHARACTERIZATION AND PHYLOGENETIC ANALYSIS OF NBS-LRR GENES IN CULTIVATED AND WILD VARIETIES OF BRINJAL (Solanum melongena L)", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 12, page no.305-309, December-2018, Available :http://www.jetir.org/papers/JETIR1812644.pdf

"MOLECULAR CHARACTERIZATION AND PHYLOGENETIC ANALYSIS OF NBS-LRR GENES IN CULTIVATED AND WILD VARIETIES OF BRINJAL (Solanum melongena L)", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp305-309, December-2018, Available at : http://www.jetir.org/papers/JETIR1812644.pdf