• Mythily S.

Soil sample was collected from the salt pan of Tuticorin and were isolated through enrichment plating technique. Five morphologically different colonies were selected from 5% salt concentration agar plate. Among the isolated bacteria, one bacterium produced pigmented colony. All the 5 isolates were subjected to gram staining and that most of the isolates were gram negative rod shaped except TSP3 which was gram positive and pleomorphic. Among all the isolates, TSP1, 2, 4 and 5 were gram negative rod shaped. The bacteria were identified as Halobacterium sp., Pseudomonas sp., Bacillus sp., Escherichia coli, and Micrococcus sp. Plasmid profile reveals that existence of a12 Kbp size plasmid in soil extract which is not isolated from cultured bacteria. The plasmid profiling among isolates exhibited the presence of different size of plasmid which showed significant difference of non cultrablebacteria.PCR amplicon of 16sr DNA isolated from pigmented bacterialphylogeny revealed that the culturable bacteria belong to Halobacterium sp. The present study concludes the salt pan contain some non culturable bacterial strain.

Salt tolerance, Plasmid profiling, metagenome, Halobacteriumsp,

"Plasmid profiling of Extremophilic Bacteria isolated from Salt Pan Ecosystem", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 12, page no.1218-1223, December-2018, Available :http://www.jetir.org/papers/JETIR1812D75.pdf

"Plasmid profiling of Extremophilic Bacteria isolated from Salt Pan Ecosystem", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp1218-1223, December-2018, Available at : http://www.jetir.org/papers/JETIR1812D75.pdf