• Usha Devi

Raw bovine milk provides whey proteins and peptides appreciated for their nutrition and a range of beneficial properties, such as those that defend from oxidative stress, lower high blood pressure, protect against microorganisms, and boost immunity. Because interest in functional foods and nutraceuticals is rising globally, there is a strong demand for techniques ensuring bioactive compounds are not lost during isolation. This study aims to assess different modern and older methods of separating bioactive whey proteins and peptides, aiming for maximum yield, high purity, and good function. Ultra-filtration, enzymatic hydrolysis, ion-exchange chromatography, and hybrid methods involving membrane chromatography and continuous foam separation were reviewed in the paper. Although traditional techniques offer a basic concept, issues like protein denaturation, polluted membranes, and difficult processing keep factories and industries from using them. Different examples and technological developments show how process optimization can resolve these barriers. The use of whey bioactives in functional foods, nutrition for medical purposes, and dietary supplements is looked at, highlighting their benefits for health and the prevention of diseases. The research ends by outlining future paths for creating sustainable and less-costly isolation strategies for functional dairy ingredients to meet the rising needs of the global market.

whey proteins, peptides, bovine milk, bioactivity, isolation techniques, functional foods, nutraceuticals, enzymatic hydrolysis, ultrafiltration

"OPTIMIZED ISOLATION TECHNIQUES FOR BIOACTIVE WHEY PROTEINS AND PEPTIDES FROM RAW BOVINE MILK", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 12, page no.80-88, December-2018, Available :http://www.jetir.org/papers/JETIR1812F69.pdf

"OPTIMIZED ISOLATION TECHNIQUES FOR BIOACTIVE WHEY PROTEINS AND PEPTIDES FROM RAW BOVINE MILK", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp80-88, December-2018, Available at : http://www.jetir.org/papers/JETIR1812F69.pdf