• Pawan Kumar Ahirwar
• S.P.Mishra
• Pramod Kumar
• I.P. Tripathi
• Ravindra Singh

- Herbal medicines are significant and reliable sources for treating various infectious and non infectious diseases. The infectious diseases create health problem, mainly in developing countries. Microorganism has developed resistance to many antibiotics and this created major problem in the treatment of infectious disease. Holarrhena antidysenterica (Roth) wall. ex A.DC. and Wrightia tomentosa Roem. et Schulta is a very significant herbal drug in Unani system of medicine and Ayurvedic system of medicine to treat various infectious diseases. In the present work done on the preliminary phytochemical studies and antibacterial activity of leaf of Holarrhena antidysentrica (HAL) and Wrightia tomentosa (WTL) in aqueous, methanol and chloroform extracts. The leaf of H. antidysentrica showed the presence of alkaloids, carbohydrate, proteins, resins and steroid but W. tomentosa leaf revealed the presence of carbohydrate, resins, flavonoids and glycoside in all extracts. The microbial limit test were carried out against Staphylococcus aureus, Salmonella sp, Pseudomonas aeruginosa, Escherichia coli, total bacterial count (TBC) and Yeast and mould (Y & M). Antibacterial activity of methanol, ethanol and acetone extracts of both plants were tested against gram positive (Staphylococcus aureus) and gram negative (Escherichia coli, Pseudomonas aeruginosa and Salmonella spp.) bacteria by agar well diffusion method along with gentamycin run parallel as a control. These parameters are helpful in identification and quality control of drugs and also used to discover natural products that may serve as lead for the development of new biomedical application.

Phytoconstituents, Antibacterial, Gentamycin, Holarrhena antidysenterica , Wrightia tomentosa.

"Preliminary phytochemical studied and antibacterial activity of Holarrhena antidysenterica (Roth) Wall. ex A.DC. and Wrightia tomentosa Roem. et Schulta.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 1, page no.9-16, January-2019, Available :http://www.jetir.org/papers/JETIR1901002.pdf

"Preliminary phytochemical studied and antibacterial activity of Holarrhena antidysenterica (Roth) Wall. ex A.DC. and Wrightia tomentosa Roem. et Schulta.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 1, page no. pp9-16, January-2019, Available at : http://www.jetir.org/papers/JETIR1901002.pdf