• BHANU RAJ MEENA
• KANIKA SHARMA

In this study antifungal activity of crude and partially purified extracts of Thevetia peruviana. Leaf extract has been assayed against Alternaria solani which is responsible for early blight of potato. Cold and hot extraction of leaf extract was prepared in different organic solvents, which were subsequently recycled by rotary vacuum evaporator. Antifungal activity of prepared plant extracts were determined by poison food technique. In cold extraction highestpercent extractive value was obtained with 100% aqueous extract and in hot extraction highest percent extractive value was obtained with 100% aqueous extract. In antifungal activity 100% alcohol crude extract and partial purified alcohol extract was show Maximum inhibition of alternaria solani. Mancozeb and bavistin were used as standards. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of alcohol fraction of Thavatia peruviana leaf extract was investigated against Alternaria solani. Results suggest that Thevetia peruviana. Leaf extract can be used to develop a bio control agent against Alternaria solani. The antifungal activity of the Thevetia peruviana due to the presence of various secondary metabolites. Hence, this plant can be used to discover bioactive natural products that may serve as leads in the development of new pharmaceuticals research activities.

"Evaluation of antifungal activity of crude and partially purified fractions of Thevetia peruviana against Alternaria solani", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 1, page no.346-355, January-2019, Available :http://www.jetir.org/papers/JETIR1901844.pdf

"Evaluation of antifungal activity of crude and partially purified fractions of Thevetia peruviana against Alternaria solani", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 1, page no. pp346-355, January-2019, Available at : http://www.jetir.org/papers/JETIR1901844.pdf