• Anjum Afshan
• Dr Md Niamat Ali
• Dr Farooz Ahmed Bhat

Abstract: Di-n-butyl phthalate (DnBP) is a manufactured chemical, commonly used as a plasticizer. It is a ubiquitous environmental contaminant. It was listed as priority pollutant by US Environmental Protection Agency. The present study aimed at determining the acute toxicity (LC50 Value) and histopathological impact of DnBP on C.carassius. The acute toxicity assay was carried out according to the standard methods in APHA and the value was assessed using the Probit Analysis method. The fish models were acclimatized to the laboratory conditions for a period of 14 days. The stock solution of DnBP was prepared and fishes were exposed to various doses ranging from 2-22 ppm for 96hrs. The result confirmed that the median lethal dose (LC50) of DnBP for the fish, Carassius carassius is 7.77 ppm. Signs of abnormal behavior were also noticed during the test such as loss of equilibrium, erratic swimming, lethargy and motionlessness. The study concluded that DnBP is highly toxic to fish, Carassius carassius with the evidence of behavioral deformations. Several degenerative changes in the histology of liver and gill tissues exposed to DnBP were noticed. Liver showed various circulatory deformities (hyperaemia, blood congestion and sinusoidal dilatation) and vacuolization of hepatocytes, whereas Gills showed hyperaemia, epithelial lifting, oedema, talengiectasia, epithelial hyperplasia and fusion of secondary lamellae. Our study concluded that exposure of C.carassius to DnBP results in tissue morphological changes. The study also described that the gills were more affected than the liver probably because of their direct contact with DnBP.

Keywords- DnBP, Acute toxicity, LC50, Probit analysis, Histopathology, C.carassius

"Assessment of Acute Toxicity and Histopathological Impact Of Di-n-Butyl Phthalate (DnBP) On Fresh Water Cyprinid Fish Crucian Carp (Carassius Carassius L.) ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 1, page no.11-25, January-2019, Available :http://www.jetir.org/papers/JETIR1901B03.pdf

"Assessment of Acute Toxicity and Histopathological Impact Of Di-n-Butyl Phthalate (DnBP) On Fresh Water Cyprinid Fish Crucian Carp (Carassius Carassius L.) ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 1, page no. pp11-25, January-2019, Available at : http://www.jetir.org/papers/JETIR1901B03.pdf