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January-2019
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Journal of Emerging Technologies and Innovative Research ( An International Scholarly Open Access Journal, Peer-reviewed, Refereed, Crossref DOI Journal )
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UGC Approved Journal no 63975(19)
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ISSN: 2349-5162 | ESTD Year : 2014 Volume 13 | Issue 3 | March 2026
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ISSN: 2349-5162 | ESTD Year : 2014 Volume 13 | Issue 3 | March 2026
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UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975
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JETIR1901F46
Registration ID:
315605
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Title
DEVELOPMENT AND VALIDATION OF OPICAPONE IN HUMAN PLASMA USING TOLCAPONE AS INTERNAL STANDARD BY LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY
Abstract
The present LC-MS/MS method for the estimation of Opicapone in human plasma by using Tolcapone as an internal standard was established and validated as per FDA guidelines The retention time of Opicapone and Tolcapone (Internal standard) was found to be 1.81 min and 2.1 min respectively. Linearity was established for Opicapone in the range of 40 ng/mL to 1800 ng/mL with correlation coefficient (r=0.999) and the overall percentage recovery was 94.63 % for Opicapone and 91.94 % for Tolcapone (Internal standard) respectively. The CV % values of accuracy and precision for Opicapone were found to be ≤ 15 %, which indicate accuracy and precision of the proposed method. The CV % values of accuracy and precision of Opicapone for stability studies were found to be ≤ 15 %, which indicate stability of the proposed method. The LC-MS/MS method for the estimation of Opicapone in human plasma by using Tolcapone as an internal standard exhibited excellent performance in terms of selectivity, linearity, accuracy, precision, recovery, stability and matrix effect test. In addition, the reported method has a short analysis run time, an advantage over previously reported methods. Therefore, this method is suitable for therapeutic drug monitoring of Opicapone.
Key Words
Opicapone, Tolcapone, Linearity, Liquid Chromatography-Tandem Mass Spectrometry.
Cite This Article
"DEVELOPMENT AND VALIDATION OF OPICAPONE IN HUMAN PLASMA USING TOLCAPONE AS INTERNAL STANDARD BY LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 1, page no.1075-1089, January-2019, Available :http://www.jetir.org/papers/JETIR1901F46.pdf
ISSN
2349-5162 | Impact Factor 7.95 Calculate by Google Scholar
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Cite This Article
"DEVELOPMENT AND VALIDATION OF OPICAPONE IN HUMAN PLASMA USING TOLCAPONE AS INTERNAL STANDARD BY LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 1, page no. pp1075-1089, January-2019, Available at : http://www.jetir.org/papers/JETIR1901F46.pdf
Publication Details
Published Paper ID: JETIR1901F46
Registration ID: 315605
Published In: Volume 6 | Issue 1 | Year January-2019
Page No: 1075-1089
Country: -, -, India .
Area: Engineering
ISSN Number: 2349-5162
Publisher: IJ Publication
Published Paper URL :: https://www.jetir.org/view?paper=JETIR1901F46
Published Paper PDF: https://www.jetir.org/papers/JETIR1901F46
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