• M.V. BALASUBRAMANYAM

Enzymes form integral part and play decisive role in biological metabolism and systems. The present studies delve on the role of enzymes viz., invertase, amylase, glucose oxidase and catalase in nectar-honey transformation during January 2018 to December 2018. Five stages in formation honey from nectar comprises floral nectar (fn), honey crop of foragers (hf), honey crop of house bees (hh), unsealed honey cells (uh) and sealed honey cells (sh). Invertase in fn and sh cells was minimum of (0.00ml) and maximum of (42.40 ml) respectively. Similarly amylase in the fn was least (0.00ml) and highest in sh (16.01 ml). Glucose oxidase in fn and sh cells was minimum of (0.00ml) and maximum of (6.68 ml) respectively. Correspondingly, catalase in the fn was least (0.00ml) and highest in sh (4.96 ml). The analysis of variance of invertase and amylase was significant at 1 % level (p<0.01), whereas glucose oxidase and catalase was not significant at 1 % level (p<0.01). Results have confirmed that the sources of all enzymes are instigated from hypopharyngeal, post-cerebral, thoracic, labial and mandibular glands of foragers and house bees are accountable for honey formation. Further, each enzyme is substrates specific which are discussed in ensuing paper.

A.dorsata indica, amylase, catalase, glucose oxidase, invertase, honey ripening.

"STUDIES ON DIFFERENT ENZYMES IN NECTAR TO HONEY TRANSFORMATIONS IN INDIGENOUS ROCKBEE, APIS DORSATA F", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 1, page no.156-164, January-2019, Available :http://www.jetir.org/papers/JETIR1901J26.pdf

"STUDIES ON DIFFERENT ENZYMES IN NECTAR TO HONEY TRANSFORMATIONS IN INDIGENOUS ROCKBEE, APIS DORSATA F", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 1, page no. pp156-164, January-2019, Available at : http://www.jetir.org/papers/JETIR1901J26.pdf