• Ansari Yaasir Ahmed Abdul Razzaq
• Dr. Sumer Singh
• Dr. Majaz Quazi
• Jameel Ahemad
• Ansari Mohd. Razi

A new HPLC method has been developed and validated with different parameters for Atenolol and Nifedipine in combine dosage form. The chromatograms were developed using a mobile phase of MeOH: OPA (70:30) with a flow rate of 0.7 ml/min. C18 Column of 4.6 x 250 mm dimension was used as a stationary phase, particle size 5µm. The detection was carried out at 233 nm. The method was validated according to ICH guidelines for linearity, precision, Repeatability, LOD and LOQ. The response was found to be linear in concentration range of 1-5 mcg/ml for Nifedipine and 20-100 mcg/ml for Atenolol. The LOD and LOQ were found to be 0.1415 and 0.4289 respectively for Atenolol and 0.1834 and 0.5558 respectively for Nifedipine. The developed method was simple, precise, accurate and reproducible and therefore suitable for routine analysis of drugs in tablet dosage form. The stability study also done through exposure of analyte solution to five different stress conditions.

HPLC, Atenolol, Nifedipine, Development, Validation.

"STABILITY INDICATING HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATIONS OF ATENOLOL AND NIFEDIPINE IN BULK AND TABLET DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 2, page no.29-36, February-2019, Available :http://www.jetir.org/papers/JETIR1902205.pdf

"STABILITY INDICATING HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATIONS OF ATENOLOL AND NIFEDIPINE IN BULK AND TABLET DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 2, page no. pp29-36, February-2019, Available at : http://www.jetir.org/papers/JETIR1902205.pdf